Bovine Viral Diarrhea Virus Type 2 (BVDV-2) MAb Ascites E2 gp53 IgG2a Isotype
BA-2
Binds to the E2 (gp53) protein of Bovine Viral Diarrhea Virus Type 2 (BVDV-2). Reacts primarily with BVDV-2 viruses. IgG2a isotype. Can be used as a typing reagent for BVDV-2 using indirect immunofluoresence or indirect immunoperoxidase reactions. This monoclonal antibody has been reported to be reactive in Western blots. This monoclonal antibody is produced as mouse ascites fluid, clarified by centrifugation, and filtered through a 0.2 micrometer filter. The concentration is 1.0 mg/ml in phosphate-buffered saline, preserved with sodium azide. Note: BVDV-1 genotypes usually produce only mild diarrhea in immunocompetent cattle, while BVDV-2 genotypes can produce thrombocytopenia, hemorrhages, and acute fatal disease. The viruses of either genotype may exist as one of two biotypes, cytopathic or noncytopathic.
SHELF LIFE:
One year from date of sale
STORAGE:
Store at 2-7°C. Do not freeze!
SIZE:
0.1 mg
ORIGIN:
Mouse Ascites
INFECTIOUS AGENT:
bovine viral diarrhea
RESTRICTIONS:
NA
KNOWN APPLICATIONS:
Immunofluorescence, Indirect immunoperoxidase
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Deregt, D., and S. Prins. A monoclonal antibody-based immunoperoxidase monolayer (micro-isolation) assay for detection of type 1 and type 2 bovine viral diarrhea viruses. Can. J. Vet. Res. 62(2):152-155 (Apr. 1998).
Deregt, D., et al. Monoclonal antibodies to the E2 protein of a new genotype (type 2) of bovine viral diarrhea virus define three antigenic domains involved in neutralization. Virus Res. 57(2):171-181 (Oct. 1998).
