VMRD has partnered with CWD Evolution to manufacture key reagents for detection of abnormal prions (PrP) by real-time quaking-induced conversion (RT-QuIC).
RT-QuIC is a next generation testing technology that fundamentally alters the way we test for CWD.
Similar to real-time PCR for identification of DNA/RNA from bacteria or viruses, RT-QuIC logarithmically amplifies abnormal prions to a detectable level.
- Ready-to-use reagents – no filtration or centrifugation required
- High-throughput – can be performed in 96- or 384-well plates
- Removal of soluble prion protein not required
- No spontaneous conversion of protein
Chronic Wasting Disease (CWD) is a rapidly spreading problem among wild and captive cervids. In the US, state and federal agencies are actively seeking new and effective ways to control this fatal disease.
RT-QuIC testing offers great potential to address key issues in CWD research & management:
Interested in learning more?
Cooper, Sarah K et al. “Detection of CWD in cervids by RT-QuIC assay of third eyelids.” PloS one vol. 14,8 e0221654. 28 Aug. 2019, doi:10.1371/journal.pone.0221654
Tewari, Deepanker et al. “Detection of chronic wasting disease in feces and recto-anal mucosal associated lymphoid tissues with RT-QuIC in a naturally infected farmed white-tailed deer herd.” Frontiers in veterinary science vol. 9 959555. 13 Sep. 2022, doi:10.3389/fvets.2022.959555>
Henderson, Davin M et al. “Progression of chronic wasting disease in white-tailed deer analyzed by serial biopsy RT-QuIC and immunohistochemistry.” PloS one vol. 15,2 e0228327. 14 Feb. 2020, doi:10.1371/journal.pone.0228327
Haley, N J et al. “Cross-validation of the RT-QuIC assay for the antemortem detection of chronic wasting disease in elk.” Prion vol. 14,1 (2020): 47-55. doi:10.1080/19336896.2020.1716657
Holz, Carine L et al. “Evaluation of Real-Time Quaking-Induced Conversion, ELISA, and Immunohistochemistry for Chronic Wasting Disease Diagnosis.” Frontiers in veterinary science vol. 8 824815. 18 Jan. 2022, doi:10.3389/fvets.2021.824815
Haley, Nicholas J et al. “Chronic wasting disease management in ranched elk using rectal biopsy testing.” Prion vol. 12,2 (2018): 93-108. doi:10.1080/19336896.2018.1436925
Hoover, Clare E et al. “Pathways of Prion Spread during Early Chronic Wasting Disease in Deer.” Journal of virology vol. 91,10 e00077-17. 28 Apr. 2017, doi:10.1128/JVI.00077-17
Haley, N J et al. “Management of chronic wasting disease in ranched elk: conclusions from a longitudinal three-year study.” Prion vol. 14,1 (2020): 76-87. doi:10.1080/19336896.2020.1724754
Haley, Nicholas J, and Jürgen A Richt. “Evolution of Diagnostic Tests for Chronic Wasting Disease, a Naturally Occurring Prion Disease of Cervids.” Pathogens (Basel, Switzerland) vol. 6,3 35. 5 Aug. 2017, doi:10.3390/pathogens6030035
