VMRD has partnered with CWD Evolution to begin manufacturing the key reagent for detection of chronic wasting disease (CWD) by real-time quaking-induced conversion (RT-QuIC).
RT-QuIC is an effective, easy, and extremely sensitive method to detect chronic wasting disease.
Detection of chronic wasting disease in many tissue types including obex, lymph nodes, and rectal biopsies.
RT-QuIC reagents produced by VMRD are validated, quality controlled, and ready-to-use.
RT-QuIC is a next generation testing technology that fundamentally alters the way we test for CWD. Instead of treatment of samples to remove soluble PrP, RT-QuIC logarithmically amplifies CWD prions to a detectable level in a similar manner as PCR amplifies DNA of viruses or bacteria for detection.
High-throughput test that can be performed in 96 or 384 well plates.
RT-QuIC has been shown to be effective for live animal testing using RAMALT tissue sample in multiple peer reviewed publications.
RT-QuIC testing offers new potential to solve the difficult problems surrounding a CWD outbreak.
CWD is a rapidly spreading disease of wild cervids in the United States. State agencies across the country are looking to develop new ways to combat this terrible disease.
RT-QuIC testing can provide numerous beneficial research projects that can make a difference in our knowledge of where CWD is found and what are the fundamental methods by which it is spreading.
Active research projects with RT-QuIC are making progress on live animal testing methods, easier hunter harvest detection methods, and increased sensitivity for post-mortem testing.
Interested in learning more?
Cooper, Sarah K et al. “Detection of CWD in cervids by RT-QuIC assay of third eyelids.” PloS one vol. 14,8 e0221654. 28 Aug. 2019, doi:10.1371/journal.pone.0221654
Tewari, Deepanker et al. “Detection of chronic wasting disease in feces and recto-anal mucosal associated lymphoid tissues with RT-QuIC in a naturally infected farmed white-tailed deer herd.” Frontiers in veterinary science vol. 9 959555. 13 Sep. 2022, doi:10.3389/fvets.2022.959555
Henderson, Davin M et al. “Progression of chronic wasting disease in white-tailed deer analyzed by serial biopsy RT-QuIC and immunohistochemistry.” PloS one vol. 15,2 e0228327. 14 Feb. 2020, doi:10.1371/journal.pone.0228327
Haley, N J et al. “Cross-validation of the RT-QuIC assay for the antemortem detection of chronic wasting disease in elk.” Prion vol. 14,1 (2020): 47-55. doi:10.1080/19336896.2020.1716657
Holz, Carine L et al. “Evaluation of Real-Time Quaking-Induced Conversion, ELISA, and Immunohistochemistry for Chronic Wasting Disease Diagnosis.” Frontiers in veterinary science vol. 8 824815. 18 Jan. 2022, doi:10.3389/fvets.2021.824815
Haley, Nicholas J et al. “Chronic wasting disease management in ranched elk using rectal biopsy testing.” Prion vol. 12,2 (2018): 93-108. doi:10.1080/19336896.2018.1436925
Hoover, Clare E et al. “Pathways of Prion Spread during Early Chronic Wasting Disease in Deer.” Journal of virology vol. 91,10 e00077-17. 28 Apr. 2017, doi:10.1128/JVI.00077-17
Haley, N J et al. “Management of chronic wasting disease in ranched elk: conclusions from a longitudinal three-year study.” Prion vol. 14,1 (2020): 76-87. doi:10.1080/19336896.2020.1724754
Haley, Nicholas J, and Jürgen A Richt. “Evolution of Diagnostic Tests for Chronic Wasting Disease, a Naturally Occurring Prion Disease of Cervids.” Pathogens (Basel, Switzerland) vol. 6,3 35. 5 Aug. 2017, doi:10.3390/pathogens6030035
