Bovine - Reagents
Bovine Viral Diarrhea Virus Type 1&2 (BVDV-1&2) MAb E2 gp53 IgG1 Isotype
One year from date of sale
Store at 2-7 °C. Do not freeze!
Binds to the E2 (gp53) protein of Bovine Viral Diarrhea Virus Type 1 (BVDV-1) and to Bovine Viral Diarrhea Virus Type 2 (BVDV-2). IgG1 isotype. Can be used as a reagent to detect both BVDV-1 and BVDV-2 using indirect immunofluoresence or indirect immunoperoxidase reactions. Does not work with formalin-fixed tissues. This monoclonal antibody is produced as mouse ascites fluid, clarified by centrifugation, and filtered through a 0.2 micrometer filter. The concentration is 1.0 mg/ml in phosphate-buffered saline, preserved with sodium azide. This antibody is stabilized by the addition of 4 mg/ml BSA.
Note: BVDV-1 genotypes usually produce only mild diarrhea in immunocompetent cattle, while BVDV-2 genotypes can produce thrombocytopenia, hemorrhages, and acute fatal disease. The viruses of either genotype may exist as one of two biotypes, cytopathic or noncytopathic.
- Deregt, D., et al. Mapping of a type 1-specific and a type-common epitope on the E2 (gp53) protein of bovine viral diarrhea virus with neutralization escape mutants. Virus Research 53(1):81-90 (Jan. 1998).
- Deregt, D., and S. Prins. A monoclonal antibody-based immunoperoxidase layer (micro-isolation) assay for detection of type 1 and type 2 bovine viral diarrhea viruses. Can. J. Vet. Res. 62(2):152-155 (Apr. 1998).
- Deregt, D., et al. Monoclonal antibodies to the E2 protein of a new genotype (type 2) of bovine viral diarrhea virus define three antigenic domains involved in neutralization. Virus Res. 57(2):171-181 (Oct. 1998).
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