We received a USDA product license for our Caprine Arthritis-Encephalitis Virus Antibody Test Kit, cELISA This assay in my opinion is the best serodiagnostic test in the world today for CAEV infection of goats Many of them describe diagnostic tests for CAEV serology. Two hundred goat sera collected from the United States were tested by IP and the cELISA. Competitive-inhibition enzyme-linked immunosorbent assay for detection of serum antibodies to caprine

The antibody responses were monitored by ELISA (Bluetongue Virus Antibody Test Kit, c-ELISA - VMRD, Inc ., USA), agar gel immunodiffusion AGID, VMRD, Inc., USA, and serum neutralization (SN) assays (9). Sensitivity values of tests employed were estimated and compared using a bayesian approach (10). the sera tested positive on AGID only on 9 d.p.i (Fig. 1). The VMRD c-ELISA was the test which showed the highest sensitivity distribution.

Bovine Virus Diarrhea Virus (BVDV) is not as easily detected by FA in chronic cell culture infections One example is observed during 9CFR 113.51-2 testing, which requires an FA test after 3-4 weeks and 1 For maximum sensitivity when testing biological fluids for infection by BVDV, we recommend that FA test

Bartonella henselae has recently been identified as a major cause of cat scratch disease in humans.(1-3) Seropositivity of affected humans to Bartonella spp. Has been significantly associated with cat contact.(1) Both kittens and adult cats carry IFA antibodies, although it appears that kittens are most often bacteremic.(3-5) There is at least one report of canine Bartonella spp. Infection causing vegetative valvular endocarditis. This dog had a high titer to Bartonella henselae by IFA.(6) 1.     Dalton, M. J., et al. Arch. Intern. Med. 155(15):1670-1676, 1995. 2.     Bergmans, A. M., et al., J. Infect. Dis. 171(4):916-923, 1995. 3.     Demers, D. M., et al., J. Pediatr. 127(1):23-26, 1995. 4.     Ueno, H., et al., Microbiol. Immunol 39(5):339-341, 1995. 5.     Jameson, P., et al., J. Infect. Dis. 172(4):1145-1149, 1995. 6.     Breitschwerdt, E.B., et.al., J. Clin. Micro. 33(1):154-160, 1995.

Brucella canis IFA substrate slides were used in parallel with the tube agglutination test (TAT) to test Sensitivity and specificity compared to the tube agglutination test were 93% and 98%, respectively. We also recommend a specific confirmatory test, such as AGID, to confirm positive reactions.

Pour off medium; add back 10 ml of serum-free media. Pour off medium; add back 10 ml of serum-free media. 

If sera are diluted in PBS with 10% adult bovine serum, antibodies reacting to bovine constituents will Care should be taken to make sure that the adult bovine serum does not contain antibodies to the agents VMRD manufactures “Special Serum Diluting Buffer” (catalog no. SSDB-100ML) for this purpose.