Bluetongue Virus Antibody Test Kit, cELISA v2
5010.20-2
Detection of antibody to Bluetongue Virus in ruminant serum by cELISA. Does not detect antibody to Epizootic Hemorrhagic Disease (EHD). For Veterinary Use Only - Use Only As Directed
SPECIES:
SAMPLE TYPE
Serum
SENSITIVY:
100.0%
SPECIFICTY:
99.0%
SHELF LIFE:
Eighteen months from date of QC release
STORAGE:
Store at 2-8°C. Do Not Freeze!
RESTRICTIONS:
N/A
CONFIGURATION:
2 stripwell plates
FORMAT:
cELISA
TESTS:
184
ASSAY TIME:
40 minutes
Bluetongue Virus Antibody Test Kit, cELISA v2
5010.20-2
Detection of antibody to Bluetongue Virus in ruminant serum by cELISA. Does not detect antibody to Epizootic Hemorrhagic Disease (EHD). For Veterinary Use Only - Use Only As Directed
SPECIES:
SAMPLE TYPE
Serum
SENSITIVY:
100.0%
SPECIFICTY:
99.0%
SHELF LIFE:
Eighteen months from date of QC release
STORAGE:
Store at 2-8°C. Do Not Freeze!
RESTRICTIONS:
N/A
CONFIGURATION:
2 stripwell plates
FORMAT:
cELISA
TESTS:
184
ASSAY TIME:
40 minutes
VMRD’s new BTV cELISA v2 test kit brings you the same rapid and convenient features of the original version (40 minutes total incubation time) along with new features including distilled water wash.
This Bluetongue Virus Antibody Test Kit is a competitive, enzyme-linked, immunosorbent assay (cELISA). Sample serum bluetongue antibody inhibits binding of horseradish peroxidase (HRP)-labeled, bluetongue-specific monoclonal antibody to bluetongue viral antigen coated on the plastic wells. Binding of the HRP-labeled monoclonal antibody conjugate is detected by the addition of enzyme substrate and quantified by subsequent color product development. Strong color development indicates little or no blockage of HRP-labeled monoclonal antibody binding and therefore the absence of bluetongue antibody in sample sera. Weak color development due to inhibition of the monoclonal antibody binding to the antigen on the solid phase indicates the presence of bluetongue antibodies in sample sera.
About Bluetongue Virus
Bluetongue is an infectious, non-contagious, arthropodborne, viral disease of wild and domestic ruminants. In cattle it is usually a subclinical infection, while in sheep it is often characterized by acute catarrhal inflammation of mucous membranes and hyperemia of coronary bands. Degenerative changes are present in skeletal and coronary musculature,leading to weakness, prolonged convalescence and significant economic losses.
Bluetongue virus (BTV) belongs to the genus Orbivirus, family Reoviridae. Laboratory diagnosis of bluetongue is primarily established by isolation of the virus. Virus is isolated in Vero or BHK 21 cells, and its presence is confirmed by immunofluorescence. Serological methods used in diagnosis of this disease are AGID, ELISA, cELISA and immunofluorescence. Positive results confirm exposure to BTV but not necessarily carrier status.
About Bluetongue Virus
Bluetongue is an infectious, non-contagious, arthropodborne, viral disease of wild and domestic ruminants. In cattle it is usually a subclinical infection, while in sheep it is often characterized by acute catarrhal inflammation of mucous membranes and hyperemia of coronary bands. Degenerative changes are present in skeletal and coronary musculature,leading to weakness, prolonged convalescence and significant economic losses.
Bluetongue virus (BTV) belongs to the genus Orbivirus, family Reoviridae. Laboratory diagnosis of bluetongue is primarily established by isolation of the virus. Virus is isolated in Vero or BHK 21 cells, and its presence is confirmed by immunofluorescence. Serological methods used in diagnosis of this disease are AGID, ELISA, cELISA and immunofluorescence. Positive results confirm exposure to BTV but not necessarily carrier status.
Technical Assistance
Our technical support team is available to assist as needed. You can reach technical support for products at support@vmrd.com.