Bovine - Test Kits
Anaplasma Antibody Test Kit, cELISA v2
One year from date of QC release.
Store at 2-7°C. Do not freeze!
2 plates, maximum 182 samples
The Anaplasma Antibody Test Kit is a competitive, enzyme-linked, immunosorbent assay (cELISA) designed to provide results which will give guidance about the presence of Anaplasma infection in cattle. Sample serum antibodies to Anaplasma inhibit the binding of horseradish peroxidase (HRP)-labeled monoclonal antibody to the Anaplasma antigen coated on the plastic wells. Binding, or lack of binding, of the HRP-labeled monoclonal antibody conjugate is detected by the addition of enzyme substrate and quantified by subsequent color product development. Strong color development indicates little or no blockage of HRP-labeled monoclonal antibody binding and therefore the absence of antibodies to Anaplasma in the sample serum. Weak or no color development due to inhibition of the monoclonal antibody binding to the antigen on the solid phase indicates the presences of Anaplasma antibodies in the sample serum.
- Knowles, D., et al. Antibody against an Anaplasma marginale MSP5 epitope common to tick and erythrocyte stages identifies persistently infected cattle. J Clin Microbiol. 34(9):2225-2230 (Sept. 1996).
- Herrero, M.V., et al. Prospective study for the detection of Anaplasma marginale Theiler, 1911 (Rickettsiales: Anaplasmataceae) in Costa Rica. Ann. N.Y. Acad Sci. 849:226-233 (June 1998).
- Torioni de Echaide, S., et al. Detection of cattle naturally infected with Anaplasma marginale in a region of endemicity by nested PCR and a competitive enzyme-linked immunosorbent assay using recombinant major surface protein 5. J. Clin. Microbiol. 36(3):777-782 (March 1998).
- N. I. Strik, et al Characterization of Anaplasma phagocytophilum Major Surface Protein 5 and the Extent of Its Cross-Reactivity with A. marginale. Clin Vaccine Immunol. 14(3): 262-268 (March 2007).
- U. M. Dreher, et al. Serologic Cross-Reactivity between Anaplasma marginale and Anaplasma phagocytophilum Clin Diagn Lab Immunol. 12(10): 1177-1183 (October 2005).
- Torioni de Echaide, S., et al. Detection of cattle naturally infected with Anaplasma marginale in a region of endemicity by nested PCR and a competitive enzyme-linked immunosorbent assay using recombinant major surface protein 5. Erratum. J. Clin. Microbiol. 39(3):1207a (March 2001).
- Coetzee JF, et al. Comparison of the complement fixation test and competitive ELISA for serodiagnosis of Anaplasma marginale infection in experimentally infected steers. Am J Vet Res.68(8):872-8 (Aug 2007).
- Hoar BR, et al. Evaluation of sequential coinfection with Anaplasma phagocytophilum and Anaplasma marginale in cattle. J Am Vet Med Assoc. 1;233(5):742 (Sep 2008).
- Scoles, G. A., et al. Validation of an Anaplasma marginale cELISA for use in the diagnosis of A. ovis infections in domestic sheep and Anaplasma spp. in wild ungulates. Vet. Microbiol. 130: 184-190 (2008).
- Chung, C., et al. Improved diagnostic performance of a commercial Anaplasma antibody competitive enzyme-linked immunosorbent assay using recombinant major surface protein 5-glutathione S-transferase fusion protein as antigen. JVDI. 26: 61-71 (2014).
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