This Bluetongue Virus antibody Test Kit is an agar-gel immunodiffusion (AGID) assay that takes place in agarose using the 7-well standard procedure described by Pearson and Jochim (American Association of Veterinary Laboratory Diagnosticians, 22nd Annual Proceedings, 463-471, 1979). Soluble bluetongue virus antigen is placed in the center well and positive reference serum is placed in three alternating peripheral wells. Sample sera are placed in the 3 remaining wells. After incubation reference lines form between the antigen well and the reference serum wells. Sample sera, if positive, will form a line that fuses with reference lines or that deviates the positive reference lines inward near the sample well without formation of a visible line. Negative sera will neither form a line nor cause deviation of the reference lines.
Detection of antibody to Bluetongue Virus (BTV) and Epizootic Hemorrhagic Disease (EHD) virus in ruminant serum by AGID. For Veterinary Use Only - Use Only As Directed