This enzyme-linked immunosorbent assay detects antibodies to Brucella ovis. Sample serum antibodies bind to Brucella antigen coated on the plastic wells of the microtiter plate. Non-specific antibody is washed away and the plate-bound antibody captures the peroxidase conjugate. The presence of bound peroxidase conjugate is detected by the addition of an enzyme substrate and quantified by subsequent color product development. Strong color development indicates the presence of antibody to B. ovis in the sample serum. Weak or no color development indicates the absence of detectable antibody to B. ovis.
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Detection of antibodies to Brucella ovis antigen in serum by ELISA.
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