Bovine Leukemia Virus Antibody Test Kit, ELISA
284-2
Detection of antibodies to Bovine Leukemia Virus (BLV) glycoprotein 51 (gp51) in bovine sera by ELISA. For Veterinary Use Only - Use Only As Directed
SPECIES:
SAMPLE TYPE
Serum
SENSITIVY:
98.0%
SPECIFICTY:
100.0%
SHELF LIFE:
One year from date of QC release
STORAGE:
Store at 2-8°C. Do Not Freeze!
RESTRICTIONS:
N/A
CONFIGURATION:
2 stripwell plates
FORMAT:
ELISA
TESTS:
182
ASSAY TIME:
60 minutes
Bovine Leukemia Virus Antibody Test Kit, ELISA
284-2
Detection of antibodies to Bovine Leukemia Virus (BLV) glycoprotein 51 (gp51) in bovine sera by ELISA. For Veterinary Use Only - Use Only As Directed
SPECIES:
SAMPLE TYPE
Serum
SENSITIVY:
98.0%
SPECIFICTY:
100.0%
SHELF LIFE:
One year from date of QC release
STORAGE:
Store at 2-8°C. Do Not Freeze!
RESTRICTIONS:
N/A
CONFIGURATION:
2 stripwell plates
FORMAT:
ELISA
TESTS:
182
ASSAY TIME:
60 minutes
This Bovine Leukemia Virus Antibody Test Kit is an enzyme-linked, immunosorbent assay (ELISA). Sample serum antibodies bind to BLV gp51 molecules attached to the plastic wells of the microtiter plate. Binding of these serum antibodies is detected by reaction with horseradish peroxidase (HRP)-labeled affinity-purified goat antibodies to bovine immunoglobulins. Attached HRP-labeled antibodies are detected by addition of enzyme substrate and quantitated by subsequent blue color product development. Strong color development indicates the presence of antibody to BLV gp51 in the sample serum. Very weak or no color development indicates the absence of antibody to BLV gp51 in the sample serum.
About Bovine Leukosis
Enzootic Bovine Leukosis (EBL) is an infectious, non-contagious viral disease of cattle. It is caused by BLV, an oncogenic delta retrovirus, which results in proliferation of B lymphocytes. Infection with BLV may lead to persistent lymphocytosis and in some adult cattle to the development of tumors with associated signs. The spread of disease from the introduction into a herd may reach enzootic proportions. Transmission of BLV occurs between animal primarliy by transfer of B lymphocytes in blood. Trauma, use of common breeding needles, and surgical procedures are the main means of transmission. It is rarely vertically transmitted. Most BLV infections are inapparent with approximately 5% of animals developing clinical signs. AGID and ELISA tests are used to identify carrier cattle. Control programs for EBL include testing and removal of positive animals. Several European countries which have instituted eradication programs also require that imported cattle be free of BLV.
About Bovine Leukosis
Enzootic Bovine Leukosis (EBL) is an infectious, non-contagious viral disease of cattle. It is caused by BLV, an oncogenic delta retrovirus, which results in proliferation of B lymphocytes. Infection with BLV may lead to persistent lymphocytosis and in some adult cattle to the development of tumors with associated signs. The spread of disease from the introduction into a herd may reach enzootic proportions. Transmission of BLV occurs between animal primarliy by transfer of B lymphocytes in blood. Trauma, use of common breeding needles, and surgical procedures are the main means of transmission. It is rarely vertically transmitted. Most BLV infections are inapparent with approximately 5% of animals developing clinical signs. AGID and ELISA tests are used to identify carrier cattle. Control programs for EBL include testing and removal of positive animals. Several European countries which have instituted eradication programs also require that imported cattle be free of BLV.
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Evermann, James F et al. “Evaluation of a serum ELISA for detection of bovine leukemia viral antibodies in milk samples.” Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc vol. 31,4 (2019): 598-600. doi:10.1177/1040638719860487
Enzootic Bovine Leukosis in OIE Manual of Diagnostic Tests and Vaccines
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