Equine Infectious Anemia Virus Antibody Test Kit, ELISA
5515.01-1
Detection of antibodies to Equine Infectious Anemia Virus in equine serum by ELISA. For Veterinary Use Only - Use Only As Directed
SPECIES:
SAMPLE TYPE
Serum
SENSITIVY:
100.0%
SPECIFICTY:
100.0%
SHELF LIFE:
One year from date of QC release
STORAGE:
Store at 2-8°C. Do Not Freeze!
RESTRICTIONS:
U.S. sales restricted to approved USDA Laboratories. No international restrictions.
CONFIGURATION:
1 stripwell plate
FORMAT:
ELISA
TESTS:
94
ASSAY TIME:
35 minutes
Equine Infectious Anemia Virus Antibody Test Kit, ELISA
5515.01-1
Detection of antibodies to Equine Infectious Anemia Virus in equine serum by ELISA. For Veterinary Use Only - Use Only As Directed
SPECIES:
SAMPLE TYPE
Serum
SENSITIVY:
100.0%
SPECIFICTY:
100.0%
SHELF LIFE:
One year from date of QC release
STORAGE:
Store at 2-8°C. Do Not Freeze!
RESTRICTIONS:
U.S. sales restricted to approved USDA Laboratories. No international restrictions.
CONFIGURATION:
1 stripwell plate
FORMAT:
ELISA
TESTS:
94
ASSAY TIME:
35 minutes
VMRD’s EIA ELISA v2 test kit brings you the same rapid and convenient features of the original version (35 minutes total incubation time and no sample dilution) along with new features including ready to use conjugate that requires no preparation time and distilled water wash. Even with these improvements, the assay still has 100% sensitivity and 100% specificity which allows detection of “weak samples.”
This Equine Infectious Anemia Virus Antibody Test Kit, ELISA is an enzyme-linked immunosorbent assay (ELISA). It is unusual in that it is neither an indirect nor a competitive ELISA. Rather, we refer to it as a modified sandwich ELISA. This format has the species agnosticism of a cELISA, and possibly improves on the inherent specificity of a cELISA, without being hampered by the single-epitope limitations of a cELISA. Equine serum is incubated in the EIAV p26 Antigen-Coated Plates and removed by dumping. Bound antibody is detected by adding Antigen-Peroxidase Conjugate (purified recombinant EIAV p26 antigen conjugated to HRP; the same antigen used to coat wells) followed by washing and Substrate Solution addition. After incubation, Stop Solution is added, changing the color of the Positive Control and any positive serum samples from blue to yellow. The results can be read using a microplate reader or visually by comparison with the Positive Control. This test for antibodies to EIAV is rapid (~35 min), yet provides high specificity and sensitivity.
EIA Reference Serum
VMRD offers EIA positive reference sera. These equine origin sera contain a level of antibody that gives a strong, medium or weak positive reaction in VMRD’s EIA ELISA. Each vial of serum comes complete with a certificate of analysis which includes a photograph of the reaction in AGID as well as the optical densities of the ELISA reaction as run in the VMRD laboratory. These reference sera are intended as reference samples for quality assurance of EIA ELISA tests.
EIA Reference Serum
VMRD offers EIA positive reference sera. These equine origin sera contain a level of antibody that gives a strong, medium or weak positive reaction in VMRD’s EIA ELISA. Each vial of serum comes complete with a certificate of analysis which includes a photograph of the reaction in AGID as well as the optical densities of the ELISA reaction as run in the VMRD laboratory. These reference sera are intended as reference samples for quality assurance of EIA ELISA tests.
Technical Assistance
Our technical support team is available to assist as needed. You can reach technical support for products at support@vmrd.com.