Mycobacterium avium subspecies paratuberculosis Antibody Test Kit, ELISA
5064.20-2
Detection of antibodies to Mycobacterium avium subsp. paratuberculosis (MAP) antigen in bovine sera, bovine milk, and caprine sera by ELISA. For Veterinary Use Only - Use Only As Directed
SPECIES:
SAMPLE TYPE
Bovine serum & milk, caprine serum
SENSITIVY:
93.11% (Bovine serum); 84.8% (Bovine milk); 100% (Caprine serum)
SPECIFICTY:
90% (Bovine serum); 82.1% (Bovine milk); 95.5% (Caprine serum)
SHELF LIFE:
Twelve months from date of QC release
STORAGE:
Store at 2-8°C. Do Not Freeze!
RESTRICTIONS:
Must be approved by State Veterinarian
CONFIGURATION:
2 stripwell plates
FORMAT:
ELISA
TESTS:
184
ASSAY TIME:
70 minutes (75 minutes w/automation)
Mycobacterium avium subspecies paratuberculosis Antibody Test Kit, ELISA
5064.20-2
Detection of antibodies to Mycobacterium avium subsp. paratuberculosis (MAP) antigen in bovine sera, bovine milk, and caprine sera by ELISA. For Veterinary Use Only - Use Only As Directed
SPECIES:
SAMPLE TYPE
Bovine serum & milk, caprine serum
SENSITIVY:
93.11% (Bovine serum); 84.8% (Bovine milk); 100% (Caprine serum)
SPECIFICTY:
90% (Bovine serum); 82.1% (Bovine milk); 95.5% (Caprine serum)
SHELF LIFE:
Twelve months from date of QC release
STORAGE:
Store at 2-8°C. Do Not Freeze!
RESTRICTIONS:
Must be approved by State Veterinarian
CONFIGURATION:
2 stripwell plates
FORMAT:
ELISA
TESTS:
184
ASSAY TIME:
70 minutes (75 minutes w/automation)
This enzyme-linked immunosorbent assay (ELISA) detects antibodies in caprine serum, bovine serum and bovine milk to Mycobacterium avium subsp. paratuberculosis (MAP), the causative agent of Johne's disease. Sample antibodies bind to MAP antigen attached to the plastic wells of the microtiter plate. Binding of these antibodies is detected by reaction with horseradish peroxidase (HRP)-labeled secondary antibody. Attached HRP-labeled antibodies are detected by addition of enzyme substrate and quantified by subsequent color product development. Strong color development indicates the presence of antibody to MAP in the sample. Very weak or no color development indicates the absence of antibody to MAP in the sample.
About Johne’s Disease
Johne’s disease is a chronic and highly contagious wasting disease of all ruminants caused by Mycobacterium avium ssp. paratuberculosis (MAP). Major clinical signs include weight loss, pipestream diarrhea, edema, decreased milk production, and eventually death. In the US, Johne’s disease affects: 70% of dairy herds,^1 costing the industry up to $250 million per year in production loss.^2 An estimated 5-10% of beef herds are also affected.^3 Currently, no effective vaccines or specific treatments are available, making proper diagnostic testing pivotal for management efforts.
1 USDA. National Animal Health Monitoring System (NAHMS) - Dairy, 2008.
2 Ott SL, et al. Prev Vet Med. , June 1999.
3 USDA. NAHMS - Beef, 1999.
About Johne’s Disease
Johne’s disease is a chronic and highly contagious wasting disease of all ruminants caused by Mycobacterium avium ssp. paratuberculosis (MAP). Major clinical signs include weight loss, pipestream diarrhea, edema, decreased milk production, and eventually death. In the US, Johne’s disease affects: 70% of dairy herds,^1 costing the industry up to $250 million per year in production loss.^2 An estimated 5-10% of beef herds are also affected.^3 Currently, no effective vaccines or specific treatments are available, making proper diagnostic testing pivotal for management efforts.
1 USDA. National Animal Health Monitoring System (NAHMS) - Dairy, 2008.
2 Ott SL, et al. Prev Vet Med. , June 1999.
3 USDA. NAHMS - Beef, 1999.
Technical Assistance
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