Neospora caninum Antibody Test Kit, cELISA
280-2
Detection of antibodies to Neospora caninum in bovine serum by cELISA. For Veterinary Use Only - Use Only As Directed
SPECIES:
SAMPLE TYPE
Serum
SENSITIVY:
96.0%
SPECIFICTY:
99.0%
SHELF LIFE:
Twenty-four months from date of QC release
STORAGE:
Store at 2-8°C. Do Not Freeze!
RESTRICTIONS:
N/A
CONFIGURATION:
2 stripwell plates
FORMAT:
cELISA
TESTS:
184
ASSAY TIME:
100 minutes
Neospora caninum Antibody Test Kit, cELISA
280-2
Detection of antibodies to Neospora caninum in bovine serum by cELISA. For Veterinary Use Only - Use Only As Directed
SPECIES:
SAMPLE TYPE
Serum
SENSITIVY:
96.0%
SPECIFICTY:
99.0%
SHELF LIFE:
Twenty-four months from date of QC release
STORAGE:
Store at 2-8°C. Do Not Freeze!
RESTRICTIONS:
N/A
CONFIGURATION:
2 stripwell plates
FORMAT:
cELISA
TESTS:
184
ASSAY TIME:
100 minutes
This Neospora caninum Antibody Test Kit is a competitive, enzyme-linked, immunosorbent assay (cELISA). Sample serum N. caninum antibody inhibits binding of horseradish peroxidase (HRP)-labeled N. caninum-specific monoclonal antibody to N. caninum tachyzoite antigen coated on the plastic wells. Binding of the HRP-labeled monoclonal antibody conjugate is detected by the addition of enzyme substrate and quantified by subsequent color product development. Strong color development indicates little or no blockage of HRP-labeled monoclonal antibody binding and therefore the absence of N. caninum antibody in sample sera. Weak color development due to inhibition of the monoclonal antibody binding to the antigen on the solid phase indicates the presence of N. caninum antibodies in sample sera.
About Neosporosis
Neosporosis has been identified across the world in various species, including dogs, cattle, sheep, goats, and horses. It is caused by Neospora caninum, a protozoan parasite closely related to Toxoplasma gondii. Although canids have been identified as the definitive host for N. caninum, it is not known if there are other definitive hosts. No clinical signs are noted in cows that abort due to N. caninum either prior to the abortion or post-abortion. Aborted fetuses are usually autolyzed with no
gross lesions and placentas are not retained. Abortions have been diagnosed in both heifers and cows from 3 months gestation to term. A majority (78%) of N. caninum abortions occur between 4 and 6 months gestation. This pattern of mid-gestation abortion is distinct from other diagnosed causes of infectious abortion in dairy cattle which tend to occur later in gestation. In dogs, N. caninum infection causes neuromuscular paralysis. Identification of carrier animals is based upon detection of specific antibody with serological tests while diagnosis of abortions is based upon microscopic examination of the fetus and immunohistochemistry.
About Neosporosis
Neosporosis has been identified across the world in various species, including dogs, cattle, sheep, goats, and horses. It is caused by Neospora caninum, a protozoan parasite closely related to Toxoplasma gondii. Although canids have been identified as the definitive host for N. caninum, it is not known if there are other definitive hosts. No clinical signs are noted in cows that abort due to N. caninum either prior to the abortion or post-abortion. Aborted fetuses are usually autolyzed with no
gross lesions and placentas are not retained. Abortions have been diagnosed in both heifers and cows from 3 months gestation to term. A majority (78%) of N. caninum abortions occur between 4 and 6 months gestation. This pattern of mid-gestation abortion is distinct from other diagnosed causes of infectious abortion in dairy cattle which tend to occur later in gestation. In dogs, N. caninum infection causes neuromuscular paralysis. Identification of carrier animals is based upon detection of specific antibody with serological tests while diagnosis of abortions is based upon microscopic examination of the fetus and immunohistochemistry.
Baszler, T.V., et al. Validation of a commercially available monoclonal antibody-based competitive-inhibition enzyme-linked immunosorbent assay for detection of serum antibodies to Neospora caninum in cattle. J. Clin. Microbiol. 39(11):3851-3857 (Nov. 2001).
Baszler, T.V., et al. Serological diagnosis of bovine neosporosis by Neospora caninum monoclonal antibody-based competitive inhibition enzyme-linked immunosorbent assay. J. Clin. Microbiol. 34(6):1423-1428 (June 1996).
Larson, R.L., et al. Economic considerations for diagnostic and control options for Neospora caninum-induced abortions in endemically infected herds of beef cattle. J. Am. Vet. Med. Assoc. 224(10):1597-1604 (May 2004).
Jakubek, E-B., and A. Uggla. Persistence of Neospora caninum-specific immunoglobulin G antibodies in bovine blood and lung tissue stored at room temperature. J. Vet. Diagn. Invest. 17(5):458-460 (Sept. 2005).
Waldner, C.L., et al. Agreement between three serological tests for Neospora caninum in beef cattle. J Vet Diagn Invest. 2004 Jul;16(4):313-5.
Millan J., et al. Seroprevalence of Toxoplasma gondii and Neospora caninum in feral cats (Felis silvestris catus) in Majorca, Balearic Islands, Spain. Vet Parasitol. 2009 Nov 12;165(3-4):323-6. Epub 2009 Jul 15
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