Small Ruminant Lentivirus Antibody Test Kit, cELISA
289-2
Detection of antibodies to gp135 of Caprine Arthritis-Encephalitis Virus (CAEV) in caprine serum and of Ovine Progressive Pneumonia Virus (OPPV) in ovine serum by cELISA. For Veterinary Use Only - Use Only As Directed
SPECIES:
SAMPLE TYPE
Serum
SENSITIVY:
100% (Caprine); 95% (Ovine)
SPECIFICTY:
99.6% (Caprine); 98.4% (Ovine)
SHELF LIFE:
Eighteen months from date of QC release
STORAGE:
Store at 2-8°C. Do Not Freeze!
RESTRICTIONS:
N/A
CONFIGURATION:
2 stripwell plates
FORMAT:
cELISA
TESTS:
184
ASSAY TIME:
110 minutes
Small Ruminant Lentivirus Antibody Test Kit, cELISA
289-2
Detection of antibodies to gp135 of Caprine Arthritis-Encephalitis Virus (CAEV) in caprine serum and of Ovine Progressive Pneumonia Virus (OPPV) in ovine serum by cELISA. For Veterinary Use Only - Use Only As Directed
SPECIES:
SAMPLE TYPE
Serum
SENSITIVY:
100% (Caprine); 95% (Ovine)
SPECIFICTY:
99.6% (Caprine); 98.4% (Ovine)
SHELF LIFE:
Eighteen months from date of QC release
STORAGE:
Store at 2-8°C. Do Not Freeze!
RESTRICTIONS:
N/A
CONFIGURATION:
2 stripwell plates
FORMAT:
cELISA
TESTS:
184
ASSAY TIME:
110 minutes
This Small Ruminant Lentivirus (SRLV) Antibody Test Kit is a competitive, enzyme-linked, immunosorbent assay (cELISA). Sample serum CAEV or OPPV antibody inhibits binding of horseradish peroxidase (HRP)-labeled SRLV-specific monoclonal antibody to CAEV antigen coated on the plastic wells. Binding of the HRP-labeled monoclonal antibody conjugate is detected by the addition of enzyme substrate and quantified by subsequent color product development. Strong color development indicates little or no blockage of HRP-labeled monoclonal antibody binding and therefore the absence of CAEV or OPPV antibody in sample sera. Weak color development due to inhibition of the monoclonal antibody binding to the antigen on the solid phase indicates the presence of CAEV or OPPV antibodies in sample sera.
About CAE and OPP
CAE and OPP (also known as maedi-visna) are persistent lentivirus infections of goats and sheep, respectively. Molecular analysis indicates that these viruses are very similar and they are often grouped together as small ruminant lentivirus (SRLV). Polyarthritis is the main clinical sign of CAEV infection, while OPP typically manifests with labored breathing and emaciation caused by progressive pneumonitis. Most SRLV-infected sheep and goats show no clinical disease, but remain persistent carriers of the virus. The major mode of viral transmission is vertically through milk and colostrum. Respiratory secretions and feces also harbor infectious virus. Good management practices, supported by a reliable diagnostic tool, are the best means of controlling the spread of disease.
About CAE and OPP
CAE and OPP (also known as maedi-visna) are persistent lentivirus infections of goats and sheep, respectively. Molecular analysis indicates that these viruses are very similar and they are often grouped together as small ruminant lentivirus (SRLV). Polyarthritis is the main clinical sign of CAEV infection, while OPP typically manifests with labored breathing and emaciation caused by progressive pneumonitis. Most SRLV-infected sheep and goats show no clinical disease, but remain persistent carriers of the virus. The major mode of viral transmission is vertically through milk and colostrum. Respiratory secretions and feces also harbor infectious virus. Good management practices, supported by a reliable diagnostic tool, are the best means of controlling the spread of disease.
Herrmann, L.M., et al. Competitive-inhibition enzyme-linked immunosorbent assay for detection of serum antibodies to caprine arthritis-encephalitis virus: Diagnostic tool for successful eradication. Clin. Diagn. Lab. Immunol. 10(2):267-271 (March 2003).
Herrmann, L.M., et al. Detection of serum antibodies to ovine progressive pneumonia virus in sheep by using a caprine arthritis-encephalitis virus competitive-inhibition enzyme-linked immunosorbent assay. Clin. Diagn. Lab. Immunol. 10(5):862-5 (September 2003).
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