DESCRIPTION

Binds to the E2 (gp53) protein of type 1 Bovine Viral Diarrhea Virus (BVDV-1). This monoclonal antibody binds specifically to BVDV-1, and not BVDV-2. IgG_2a isotype. Can be used as a typing reagent for BVDV-1 using indirect immunofluorescence or indirect immunoperoxidase reactions. This monoclonal antibody has also been used in virus neutralization assays. This monoclonal antibody is produced as mouse ascites fluid, clarified by centrifugation, and filtered through a 0.2 micrometer filter. The concentration is 1.0 mg/ml in phosphate-buffered saline, preserved with sodium azide.

Note: BVDV-1 genotypes usually produce only mild diarrhea in immunocompetent cattle, while BVDV-2 genotypes can product thrombocytopenia, hemorrhages, and acute fatal disease. The viruses of either genotype may exist as one of two biotypes, cytopathic or noncytopathic.

REFERENCES

• Deregt, D., et al. Mapping of a type 1-specific and a type-common epitope on the E2 (gp53) protein of bovine viral diarrhea virus with neutralization escape mutants. Virus Res. 53(1):81-90 (Jan. 1998).
• Deregt, D., and S. Prins. A monoclonal antibody-based immunoperoxidase monolayer (micro-isolation) assay for detection of type 1 and type 2 bovine viral diarrhea viruses. Can. J. Vet. Res. 62(2):152-155 (Apr. 1998).
• Deregt, D., et al. Monoclonal antibodies to the E2 protein of a new genotype (type 2) of bovine viral diarrhea virus define three antigenic domains involved in neutralization. Virus Res. 57(2):171-181 (Oct. 1998).