DESCRIPTION

The Coombs test, also called direct antiglobulin test, is designed to detect immune-mediated erythrocyte destruction which occurs in autoimmune hemolytic anemias, and in some cases with infections, neoplastic disorders canine systemic lupus erythematosis. Hemolysis in these diseases is caused by erythrocytes being coated with antibody (IgG, IgM) and/or complement components (C3). Coated erythrocytes are either lysed in the bloodstream and/or removed by phagocytes. The Coombs reagent is an antiserum to IgG, IgM, and C3 prepared in goats. After obtaining the antiserum, complement is inactivated at 56°C for 30 minutes and then the antiserum is absorbed repeatedly with washed normal canine erythrocytes. These treatments ensure that the Coombs reagent will not react with normal erythrocytes. However, erythrocytes that are coated with IgG, IgM, and/or C3 will be agglutinated by the Coombs reagent as it contains antibodies to IgG, IgM, and C3.

PRODUCT RESTRICTIONS

No Product Restrictions

REFERENCES

• Quimby FW, et al. Efficacy of immunoserodiagnostic procedures in the recognition of canine immunologic diseases.Am J Vet Res. 1980 Oct;41(10):1662-6.
• Slappendel RJ. The diagnostic significance of the direct antiglobulin test (DAT) in anemic dogs. Vet Immunol Immunopathol. 1979 Nov;1(1):49-59.