Cervine - Reagents
Chronic Wasting Disease (CWD) 5X Reaction Buffer
1.0 ml 5x RT-QuIC Reaction buffer (5x PBS pH 7.4, 0.85M NaCl, 5 mM EDTA 50 µM Thioflavin T.
VMRD RT-QuIC Reaction buffer is a tried-and-true recipe for a balance of sensitivity and specificity in RT-QuIC reactions. The buffer comes ready-to-use including thioflavin T and requires no filtering. Just add water to the 5x buffer and then VMRD RT-QuIC amplification substrate and it's ready to go.
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