Neospora caninum control strategy based on testing herds for antibody
Neospora caninum control strategy based on testing herds for antibody
  • VMRD | 2004-10-01

A survey of 93 dairy and five beef herds from five regions of the United States revealed that at least one cow in 90% of the herds was seropositive for antibody to Neospora caninum (1). The prevalence of seropositive cattle in...

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Using Conditional Formatting to Highlight Positives, or Any Sample of Interest
Using Conditional Formatting to Highlight Positives, or Any Sample of Interest
  • Ethan Adams, Editor in Chief | 2004-10-01

In the last issue (VMRD Newsletter, April 2004) we saw how to use Microsoft Excel to calculate positive / negative values from optical density (OD) results of ELISA assays. This can be helpful in reducing faulty results caused by human error and...

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CAEV:
CAEV: "The Rest of the Story"
  • D. Scott Adams, D.V.M., Ph.D., President and CEO | 2004-04-01

To the Irish March 17 was St. Patrick’s Day, but for me it was a major milestone for VMRD and for me personally. We received a USDA product license for our Caprine Arthritis-Encephalitis Virus Antibody Test Kit, cELISA, the...

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Easy Conversion of Optical Densities to Positive/Negative Results Using Microsoft Excel
Easy Conversion of Optical Densities to Positive/Negative Results Using Microsoft Excel
  • Ethan Adams, Editor in Chief | 2004-04-01

Perhaps you have long suspected that somewhere, concealed deep within the labyrinth of Microsoft Excel, lies an easy way to make your computer translate your O.D’s. into positive/negative values. Unfortunately, that little paper clip guy...

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Cattle Are a Major Reservoir of Anaplasma marginale
Cattle Are a Major Reservoir of Anaplasma marginale
  • Travis C. McGuire, D.V.M., Ph.D., Director of Research | 2004-04-01

Cattle persistently infected with Anaplasma marginale are a major reservoir for transmission of anaplasmosis.  This is because a main mode of transmission is by ticks, yet the ticks involved in transmission do not ...

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Taxonomic Nomenclature Changes
Taxonomic Nomenclature Changes
  • Ethan Adams, Editor in Chief | 2004-04-01

“What’s in a name? That which we call a rose by any other name would smell as sweet.”  It might smell as sweet, yes, but it would be much harder to remember.  ...

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The 2000 Bluetongue Outbreak in Italy
The 2000 Bluetongue Outbreak in Italy
  • Savini G., Monaco F., Calzetta G., Antonucci D., Casaccia C., Titarelli M., De Santis P., Conte A., Lelli R. | 2001-08-01

The aim of this experimental trial was to study viraemia and immune response in local breeds of sheep and goat, in order to evaluate their possible role in the epidemiology of bluetongue outbreak in Italy, and to assess the diagnostic...

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Lab Tip: BVDV Detection
Lab Tip: BVDV Detection
  • John W. Black, American BioResearch | 1996-04-01

Bovine Virus Diarrhea Virus (BVDV) is not as easily detected by FA in chronic cell culture infections as it is in acute infections. One example is observed during 9CFR 113.51-2 testing, which requires an FA test after 3-4 weeks and...

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Cat Scratch Disease
Cat Scratch Disease
  • VMRD | 1996-01-01

Bartonella henselae has recently been identified as a major cause of cat scratch disease in humans.1-3 Seropositivity of affected humans to Bartonella spp. Has been significantly associated with cat contact.1 Both kittens and adult...

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IFA Test for Canine Brucellosis
IFA Test for Canine Brucellosis
  • VMRD | 1995-11-01

Brucella canis IFA substrate slides were used in parallel with the tube agglutination test (TAT) to test 488 canine samples submitted to the Texas Veterinary Medical Diagnostic Laboratory (TVMDL), Serology Section. Results are listed in the table below. FITC-labeled anti-dog...

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Confirmation of Immunoglobulin Class Specificity
Confirmation of Immunoglobulin Class Specificity
  • John W. Black, American BioResearch | 1995-08-01

Heavy chain specificity may be confirmed by preincubation of sera in 0.1 M 2-Mercaptoethanol. The procedure is as follows: Dilute sera in PBS (four-fold dilutions) with and without 0.1 M 2-Mercaptoethanol through the endpoint and incubate for 30 minutes at 37°C. Incubate...

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Background Problems with IFA
Background Problems with IFA
  • VMRD | 1995-04-01

Cure for Background with IFA for Dog and Cat Viruses and Other Agents. Because of the presence of bovine origin products in vaccines, dogs and cats develop antibodies that react with cell culture origin substrates.  If sera...

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