Obtaining Quantitative Data Using VMRD's cELISAs
Obtaining Quantitative Data Using VMRD's cELISAs
  • Travis C. McGuire, D.V.M., Ph.D., Director of Research | 2005-10-01

It is often useful to know the titer of antibody in a serum for comparison with sera taken earlier or later from the same animal or for other purposes. The cELISAs made by VMRD recommend using undiluted sera and...

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Equine Piroplasmosis: Detection of Carriers Prior to Importation into the United States
Equine Piroplasmosis: Detection of Carriers Prior to Importation into the United States
  • D. Scott Adams, D.V.M., Ph.D., President and CEO | 2005-04-01

Equine piroplasmosis is a tick-borne hemoparasitic disease of horses caused byBabesia equi, B. caballi or both. Piroplasmosis is known to be endemic in every country except the USA, Canada, England, Ireland, Japan and Australia. Thus, in these countries...

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Reasons to Avoid the CF Test for Detecting Antibodies in Cattle, Sheep, Goat and Horse Sera
Reasons to Avoid the CF Test for Detecting Antibodies in Cattle, Sheep, Goat and Horse Sera
  • Travis C. McGuire, D.V.M., Ph.D., Director of Research | 2005-04-01

The complement fixation (CF) test is sometimes used for detection of antibody to infectious agents in domestic animals. There is a major problem with the use of this test to detect antibodies in serum for cattle, sheep, goats and horses...

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Canine Distemper Is Alive and Well and More Dogs Are Dead
Canine Distemper Is Alive and Well and More Dogs Are Dead
  • D. Scott Adams, VMRD, Inc., Pullman, WA, Bob Page, NeoTech, LLC, Dresden, TN, John W. Black, American BioResearch, Sevierville, TN | 2004-10-01

According to the third edition of Veterinary Virology (1999), “Canine distemper is the most important viral disease of dogs, producing high morbidity and mortality in unvaccinated populations worldwide.” However, for many years, perhaps 20 or 30, canine distemper (CD) has not...

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Neospora caninum control strategy based on testing herds for antibody
Neospora caninum control strategy based on testing herds for antibody
  • VMRD | 2004-10-01

A survey of 93 dairy and five beef herds from five regions of the United States revealed that at least one cow in 90% of the herds was seropositive for antibody to Neospora caninum (1). The prevalence of seropositive cattle in...

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Using Conditional Formatting to Highlight Positives, or Any Sample of Interest
Using Conditional Formatting to Highlight Positives, or Any Sample of Interest
  • Ethan Adams, Editor in Chief | 2004-10-01

In the last issue (VMRD Newsletter, April 2004) we saw how to use Microsoft Excel to calculate positive / negative values from optical density (OD) results of ELISA assays. This can be helpful in reducing faulty results caused by human error and...

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CAEV:
CAEV: "The Rest of the Story"
  • D. Scott Adams, D.V.M., Ph.D., President and CEO | 2004-04-01

To the Irish March 17 was St. Patrick’s Day, but for me it was a major milestone for VMRD and for me personally. We received a USDA product license for our Caprine Arthritis-Encephalitis Virus Antibody Test Kit, cELISA, the...

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Easy Conversion of Optical Densities to Positive/Negative Results Using Microsoft Excel
Easy Conversion of Optical Densities to Positive/Negative Results Using Microsoft Excel
  • Ethan Adams, Editor in Chief | 2004-04-01

Perhaps you have long suspected that somewhere, concealed deep within the labyrinth of Microsoft Excel, lies an easy way to make your computer translate your O.D’s. into positive/negative values. Unfortunately, that little paper clip guy...

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Cattle Are a Major Reservoir of Anaplasma marginale
Cattle Are a Major Reservoir of Anaplasma marginale
  • Travis C. McGuire, D.V.M., Ph.D., Director of Research | 2004-04-01

Cattle persistently infected with Anaplasma marginale are a major reservoir for transmission of anaplasmosis.  This is because a main mode of transmission is by ticks, yet the ticks involved in transmission do not ...

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Taxonomic Nomenclature Changes
Taxonomic Nomenclature Changes
  • Ethan Adams, Editor in Chief | 2004-04-01

“What’s in a name? That which we call a rose by any other name would smell as sweet.”  It might smell as sweet, yes, but it would be much harder to remember.  ...

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The 2000 Bluetongue Outbreak in Italy
The 2000 Bluetongue Outbreak in Italy
  • Savini G., Monaco F., Calzetta G., Antonucci D., Casaccia C., Titarelli M., De Santis P., Conte A., Lelli R. | 2001-08-01

The aim of this experimental trial was to study viraemia and immune response in local breeds of sheep and goat, in order to evaluate their possible role in the epidemiology of bluetongue outbreak in Italy, and to assess the diagnostic...

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Lab Tip: BVDV Detection
Lab Tip: BVDV Detection
  • John W. Black, American BioResearch | 1996-04-01

Bovine Virus Diarrhea Virus (BVDV) is not as easily detected by FA in chronic cell culture infections as it is in acute infections. One example is observed during 9CFR 113.51-2 testing, which requires an FA test after 3-4 weeks and...

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