Taxonomic Nomenclature Changes
Taxonomic Nomenclature Changes
  • Ethan Adams, Editor in Chief | 2004-04-01

“What’s in a name? That which we call a rose by any other name would smell as sweet.”  It might smell as sweet, yes, but it would be much harder to remember.  ...

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The 2000 Bluetongue Outbreak in Italy
The 2000 Bluetongue Outbreak in Italy
  • Savini G., Monaco F., Calzetta G., Antonucci D., Casaccia C., Titarelli M., De Santis P., Conte A., Lelli R. | 2001-08-01

The aim of this experimental trial was to study viraemia and immune response in local breeds of sheep and goat, in order to evaluate their possible role in the epidemiology of bluetongue outbreak in Italy, and to assess the diagnostic...

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Lab Tip: BVDV Detection
Lab Tip: BVDV Detection
  • John W. Black, American BioResearch | 1996-04-01

Bovine Virus Diarrhea Virus (BVDV) is not as easily detected by FA in chronic cell culture infections as it is in acute infections. One example is observed during 9CFR 113.51-2 testing, which requires an FA test after 3-4 weeks and...

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Cat Scratch Disease
Cat Scratch Disease
  • VMRD | 1996-01-01

Bartonella henselae has recently been identified as a major cause of cat scratch disease in humans.1-3 Seropositivity of affected humans to Bartonella spp. Has been significantly associated with cat contact.1 Both kittens and adult...

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IFA Test for Canine Brucellosis
IFA Test for Canine Brucellosis
  • VMRD | 1995-11-01

Brucella canis IFA substrate slides were used in parallel with the tube agglutination test (TAT) to test 488 canine samples submitted to the Texas Veterinary Medical Diagnostic Laboratory (TVMDL), Serology Section. Results are listed in the table below. FITC-labeled anti-dog...

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Confirmation of Immunoglobulin Class Specificity
Confirmation of Immunoglobulin Class Specificity
  • John W. Black, American BioResearch | 1995-08-01

Heavy chain specificity may be confirmed by preincubation of sera in 0.1 M 2-Mercaptoethanol. The procedure is as follows: Dilute sera in PBS (four-fold dilutions) with and without 0.1 M 2-Mercaptoethanol through the endpoint and incubate for 30 minutes at 37°C. Incubate...

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Background Problems with IFA
Background Problems with IFA
  • VMRD | 1995-04-01

Cure for Background with IFA for Dog and Cat Viruses and Other Agents. Because of the presence of bovine origin products in vaccines, dogs and cats develop antibodies that react with cell culture origin substrates.  If sera...

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Exfoliation Technique for Detection of Non-Cytopathic BVD in Cell Cultures
Exfoliation Technique for Detection of Non-Cytopathic BVD in Cell Cultures
  • John W. Black, American BioResearch | 1995-04-01

Culture cells to confluency in a 25cm2 flask. Pour off medium; add back 10 ml of serum-free media. Using a cotton swab, scrape off about half of the cell sheet and triturate with a pipette. Pour off medium; add back...

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What Are The Patterns of Fluorescence in Distemper Direct FA?
What Are The Patterns of Fluorescence in Distemper Direct FA?
  • VMRD | 1994-10-01

Two kinds of antemortem samples are most often examined; blood smears and conjunctival smears. Thin smears of whole blood or (preferably) buffy coat cells should be air dried for 30 minutes and fixed in a mixture of 25% methanol and 75% acetone...

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