Background Problems with IFA
- VMRD | 1995-04-01
Cure for Background with IFA for Dog and Cat Viruses and Other Agents. Because of the presence of bovine origin products in vaccines, dogs and cats develop antibodies that react with cell culture origin substrates. If sera...
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Exfoliation Technique for Detection of Non-Cytopathic BVD in Cell Cultures
- John W. Black, American BioResearch | 1995-04-01
Culture cells to confluency in a 25cm2 flask. Pour off medium; add back 10 ml of serum-free media. Using a cotton swab, scrape off about half of the cell sheet and triturate with a pipette. Pour off medium; add back...
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What Are The Patterns of Fluorescence in Distemper Direct FA?
- VMRD | 1994-10-01
Two kinds of antemortem samples are most often examined; blood smears and conjunctival smears. Thin smears of whole blood or (preferably) buffy coat cells should be air dried for 30 minutes and fixed in a mixture of 25% methanol and 75% acetone...
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